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plx301 construct  (Addgene inc)


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    Structured Review

    Addgene inc plx301 construct
    Plx301 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plx301+construct/pLX301+(Plasmid+%2325895)/bio_rxiv__64898__2026__03__16__712096-216-24-29
    Average 93 stars, based on 66 article reviews
    plx301 construct - by Bioz Stars, 2026-10
    93/100 stars

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    Produced:

    Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin
    Article Snippet: .. Lentivirus was produced by transfecting 1×10^6 HEK293T cells in 2 ml growth medium per well of a 6 well plate with respective 1.25 μg pLX301 construct, 900 ng psPAX2 (Addgene, 12260) and 300 ng pVSV-G (Addgene, 8454) using Lipofectamine 2000 Transfection Reagent (Invitrogen, Thermo Fisher Scientific, 11668027) according to the manufacturer’s protocol. .. In brief, DNA was diluted in 100 μl Opti-MEM (Gibco, Thermo Fisher Scientific, 11058021) and 6 μl Lipofectamine 2000 were diluted in 100 μl Opti-MEM.

    Construct:

    Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin
    Article Snippet: .. Lentivirus was produced by transfecting 1×10^6 HEK293T cells in 2 ml growth medium per well of a 6 well plate with respective 1.25 μg pLX301 construct, 900 ng psPAX2 (Addgene, 12260) and 300 ng pVSV-G (Addgene, 8454) using Lipofectamine 2000 Transfection Reagent (Invitrogen, Thermo Fisher Scientific, 11668027) according to the manufacturer’s protocol. .. In brief, DNA was diluted in 100 μl Opti-MEM (Gibco, Thermo Fisher Scientific, 11058021) and 6 μl Lipofectamine 2000 were diluted in 100 μl Opti-MEM.

    Transfection:

    Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin
    Article Snippet: .. Lentivirus was produced by transfecting 1×10^6 HEK293T cells in 2 ml growth medium per well of a 6 well plate with respective 1.25 μg pLX301 construct, 900 ng psPAX2 (Addgene, 12260) and 300 ng pVSV-G (Addgene, 8454) using Lipofectamine 2000 Transfection Reagent (Invitrogen, Thermo Fisher Scientific, 11668027) according to the manufacturer’s protocol. .. In brief, DNA was diluted in 100 μl Opti-MEM (Gibco, Thermo Fisher Scientific, 11058021) and 6 μl Lipofectamine 2000 were diluted in 100 μl Opti-MEM.



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    93
    Addgene inc plx301 construct
    Plx301 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plx301+construct/pLX301+(Plasmid+%2325895)/bio_rxiv__64898__2026__03__16__712096-216-24-29
    Average 93 stars, based on 1 article reviews
    plx301 construct - by Bioz Stars, 2026-10
    93/100 stars
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